BRCA1 Accelerates CtIP-Mediated DNA-End Resection

摘要

DNA-end resection is a highly regulated and critical\udstep in the response and repair of DNA double-strand\udbreaks. In higher eukaryotes, CtIP regulates resection\udby integrating cellular signals via its posttranslational\udmodifications and protein-protein interactions,\udincluding cell-cycle-controlled interaction with\udBRCA1. The role of BRCA1 in DNA-end resection is\udnot clear. Here, we develop an assay to study DNA\udresection in higher eukaryotes at high resolution. We\uddemonstrate that the BRCA1-CtIP interaction, albeit\udnot essential for resection, modulates the speed at\udwhich this process takes place